An analytic description of electrodynamic dispersion in free-flow zone electrophoresis

نویسندگان

چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Electrokinetic Flow and Dispersion in Capillary Electrophoresis

Electrophoretic separation of a mixture of chemical species is a fundamental technique of great usefulness in biology, health care, and forensics. In capillary electrophoresis (which has evolved from its predecessor, slab-gel electrophoresis), the sample migrates through a single microcapillary instead of through the network of pores in a gel. A fundamental design problem is to minimize dispers...

متن کامل

Free-zone electrophoresis in glass capillaries.

A simple theory of zone electrophoresis in open-tubular capillaries is developed. According to this theory, to achieve the highest resolution of zones, tubes with as small an inside diameter as possible should be used in combination with as high an applied voltage as feasible. To test this approach, we performed electrophoresis in glass capillaries with an internal diameter of 75 micron and a l...

متن کامل

Computational simulation of migration in free capillary zone electrophoresis I. Description of the theoretical model

An instrument simulator was developed for high-performance capillary electrophoresis which allows for fast graphic illustration of the effect of a large number of variables on the shape of the electropherogram. The input data of the separands are values of pK and mobilities at 25°C and infinite dilution. The instrument parameters that can be varied include capillary material, lengths, inside di...

متن کامل

Free flow electrophoresis of chloroplasts.

Highly purified intact chloroplasts were isolated from spinach (Spinacia oleracea L.) leaves by free flow electrophoresis. Morphological and biochemical studies showed that the fraction enriched in intact chloroplasts has a higher protein to chlorophyll ratio and a higher linolenic acid content than the broken organelles of the other fraction. The intact chloroplasts prepared by electrophoresis...

متن کامل

Reducing pH gradients in free-flow electrophoresis.

Small-volume continuous-flow synthesis (small-volume CFS) offers a number of benefits for use in small-scale chemical production and exploratory chemistry. Typically, small-volume CFS is followed by discontinuous purification; however, a fully continuous synthesis-purification combination is more attractive. Milli free-flow electrophoresis (mFFE) is a promising continuous-flow purification tech...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Chromatography A

سال: 2015

ISSN: 0021-9673

DOI: 10.1016/j.chroma.2015.05.035